Martens, who was named player of the year by FIFA in 2017 when the Dutch won EURO 2017, sat out training on Friday for a second successive day with a painful foot after teammate Jill Roord jumped on it when they were celebrating.
26-year-old Martens had a largely quiet tournament before bursting into the limelight on Wednesday with both goals, the second from the penalty spot, as the Dutch edged the 2011 World Cup winners 2-1 at Rennes.
But her chances of playing against Italy looked bleak after she was unable to train again on Friday, just hours after telling reporters she was confident of playing.