Michael McCormack says the party will be renewing its focus on regional Australia, after he was re-elected as leader of the Nationals party.
Mr McCormack fended off a challenge from former leader Barnaby Joyce, but did not release the results of the ballot.
"I'd like to think the entire National Party team, and I know they will now unite," Mr McCormack told reporters at Parliament House.
"The fact is, I shook hands with Barnaby. We will move on. We will move on and work hard together."
He said he has been endorsed as party leader "three times in less than two years", which should put to rest any further challenges from Mr Joyce.
"I think that is enough to warrant me leading the party going forward.""It's a great honour and a privilege to continue to serve as the leader of the National Party."
David Littleproud is the new deputy leader of the Nationals party. Source: SBS
He said it is now time to end any dispute over the leadership and put the people of regional Australia first.
"We have your back, whether it's drought, we have your back whether it's building new dam infrastructure," he said.
"Regional Australia has a bright future. We will be there to ensure the right policies and the right infrastructure's in place to realise the potential of the future.
It is the first time a Nationals leader has been challenged, prompting a party room vote, in more than 30 years.
Queenslander David Littleproud was named as the party's deputy leader.
The Natural Disaster and Emergency Management Minister said it is a huge honour to be recognised.
"This is a proud day for me."
'Shenanigans are over'
Mr Littleproud said he wanted the party to focus on serving Australians from here on out.
"The shenanigans are over, it's time to get back to looking after those people that are facing drought, that have faced up to the fires. It's time for us to focus on them, not us. The party has to focus on that.
"We've lost too many young people out of regional and rural Australia, it's time to bring them home, time for them to believe in regional."
Matt Canavan resigned as resources minister from the Morrison government Cabinet on Monday, after he backed Barnaby Joyce in the Nationals leadership spill.
"We need a bulldog, we need a fighter," he told reporters in Canberra ahead of the party room vote.
The party was forced to choose a new deputy leader to replace dumped minister Bridget McKenzie after the sports grants scandal.